: buffer, culture media 필터링 및 DNA prep, protein purification 등 여러 applications에 적용 가능
Reduction of Hanging Drops
- 새로운 outlet tip 적용으로 Hanging Drops 감소: 교차오염 가능성 최소화
Hanging drops were measured by evacuating wells of fluid, weighing the plate, and then blotting and re-weighing the plate.
Three plates of each type (350 μL well volume) were tested and the averages calculated.
Applications
- Prefiltration
- Particulate removal
- DNA prep
- Protein purification
제품사양
Materials of Construction
Filter Media: Supor (polyethersulfone) membrane Glass fiber (borosilicate glass without binder) media DNA binding (borosilicate glass without binder) media Mustang Q (anion exchange) membrane Mustang S (cation exhange) membrane Omega (modified polyethersulfone) media PP/PE non-woven (polypropylene/polyethylene) media PTFE (polytetrafluoroethylene) membrane Plate Housing: Polyproylene Lid: Polystyrene
Dimensions
Length: 12.8 cm (5.0 in.) Width: 8.6 cm (3.4 in.) Height (With Lid): 1.8 cm (0.7 in.) (350 μL only) Height (Without Lid): 350 μL: 1.4 cm (0.6 in.) 1 mL: 3.3 cm (1.3 in.) 2 mL: 4.7 cm (1.9 in.)
Well-Bottom Area
0.25 cm2
Recommended Working Volume
350 μL: ≤ 300 μL 1 mL: ≤ 900 μL 2 mL: ≤ 1.9 mL
Recommended Operating Vacuum
≥ 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
1,500 x g
Typical Hold-Up Volume
Filter plates were filled with 300 μL of water and filtered at 10 in. Hg, except plates containing PTFE membrane, which were filled with 60% isopropyl alcohol/40% water.