제품특징

Advantages

- 일반 cloning 및 발현 벡터 (pUC and pET-type vectors) 사용시 독성유전자 (toxic inserts/gene) 발현 안정화

- Unstable DNA를 가진 plasmid DNA를 보다 안정적으로 cloning 할 수 있도록 copy number를 현저하게 낮춘 후, induction solution에 의한 유도 조건에서만 copy number 높여 고효율의 DNA 획득

- Bacteriophages T1 및 T5 내성 유전자 tonA로 파지 오염 최소화

- Chemical 또는 Electrocompetent cell type 선택 가능

높은 transformation 효율

- >1 x 1010 cfu/µg of pUC19 (Electro competent) 

- >1 x 107 cfu/µg of pUC19 (Chemically competent)

Figure 1. Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter™ EPI400™ E. coli cells.

(Lanes C: TransforMax™ EC100™ cells/ Lanes U: uninduced CopyCutter EPI400 cells/ Lanes I: induced CopyCutter EPI400 cells)

동일한 수의 lysed cell(based on OD600)에서 추출된 DNA가 각 Lane에 로딩되어 진행되었습니다.

Figure 2. DNA inserts encoding toxic gene products were successfully cloned into high-copy-number vectors using CopyCutter™ EPI400™ E. coli cells. 

시퀀싱 후, TransforMAX™ EC100™ cells의 full-length acpP clones는 point mutations을 여러 개 포함하고 있는 것으로 나타났습니다.

[Genotype]

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA pcnB4 dhfr 

Applications

- Difficult cloning (Copy number control/toxic proteins)

- lacZΔM15에 의한 Blue/white screening

- [mcrA, Δ(mrr-hsdRMS-mcrBC)]에 methylated DNA의 효율적인 cloning

주문정보

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	C400CH10	CopyCutter™ EPI400™ Chemically Competent E.coli	10 x 50 µl	Lucigen	Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA		카트담기 견적문의
	C400EL10	CopyCutter™ EPI400™ Electrocompetent E.coli	10 x 50 µl	Lucigen	Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA		카트담기 견적문의
	CIS40025	CopyCutter™ Induction Solution	25 ml	Lucigen	Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA		카트담기 견적문의

	전체선택
	CopyCutter™ EPI400™ Chemically Competent E.coli Cat No. C400CH10 규격 10 x 50 µl 제조사 Lucigen 제품정보 Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA 수량 카트담기 견적문의
	CopyCutter™ EPI400™ Electrocompetent E.coli Cat No. C400EL10 규격 10 x 50 µl 제조사 Lucigen 제품정보 Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA 수량 카트담기 견적문의
	CopyCutter™ Induction Solution Cat No. CIS40025 규격 25 ml 제조사 Lucigen 제품정보 Competent cell, * C400CH10 and C400EL10 are supplied with 1 ml (1000X) CopyCutter™ Induction Solution and 10 μl (100 pg/μl) pUC19 Control DNA 수량 카트담기 견적문의

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